Seminar: Deciding the final design of the DCCC ctDNA Research Center's ctDNA reference material
Date: 22 March 2023
Time: 03:00 pm - 05:00 pm (kl. 15:00 - ca. 17:00)
Place: Online at Zoom (link will follow in an e-mail after registration deadline)
Seminar leaders: Laura Elgaard Iisager Jensen, Research Assistant, Department of Molecular Medicine (MOMA) Aarhus University and Aarhus University Hospital
Mads Heilskov Rasmussen, Cand.Scient., Ph.D., Department of Molecular Medicine (MOMA) Aarhus University and Aarhus University Hospital
Target population: Anyone in the center interested in being involved in the design of the DCCC ctDNA center’s FINAL reference material set for ctDNA detection methods.
Registration: Please register here (registration deadline is the 20th of March 2023)
OBS this seminar is for center members - working at a university or hospital in Denmark.
Background:
Circulating tumor DNA (ctDNA) is becoming a valuable tool for non-invasive cancer detection and profiling. However, effective clinical integration of ctDNA analysis requires standardization, benchmarking, and quality control of ctDNA assays. In the ctDNA center’s National Infrastructure 2 (NI2), we aim to develop stable and unlimited reference material (RefMat) for the common ctDNA detection methods, NGS and ddPCR, using artificial, mutated spike-in ctDNA. The RefMat will be available for other ctDNA laboratories to use, with the aim of achieving common standards for ctDNA detection across facilities, and a standardized protocol for optimal generation of ctDNA RefMat, in general.
Purpose:
The purpose of the workshop is:
- To plan the design of the DCCC ctDNA Center's ctDNA RefMat in relation to conduction of the planned QA ring trials and ctDNA method benchmarking experiments.
- To create the most useful, widespread, and accommodating panel of standards for all center members to use.
Agenda:
- Presentation of the RefMat protocol.
- Presentation of the draft version of RefMat spike-in mutations.
- Discussion of the RefMat design:
- Do the included mutations cover the most important aspects related to ctDNA detection such as noise level and mutation type ?
- Are the mutations relevant, e.g. have high recurrence indices, are potential drug targets, and are my favorite mutations included ?
- What allele frequency distribution should be represented ?
- Discussion of the potential use of the RefMat, specifically:
- QA ring trials.
- Method benchmarking.
- Internal laboratory control.
Preparation:
- Read the handed out preliminary RefMat design and consider possibly changes or improvements relating to your laboratory's preferences.
This will be e-mailed to you before the seminar
The Seminar Series
The Seminar is a part of a new initiative by The Danish National Center for Circulating Tumor DNA Guided Cancer Treatment; Seminar Series. The Seminar Series is a range of different seminars and workshops about ctDNA guided cancer treatment. They are open for everyone in the ctDNA Center - so feel free to share it with others who are working with or interested in ctDNA in Denmark.
The seminars will be held approximately every second month.
The Seminar Series is an element of the ctDNA Research Center’s aim to facilitate national collaboration regarding research on ctDNA guided cancer treatment.
Everyone in the center can contribute with a workshop in their field of expertise or suggest wishes for a theme for a seminar - contact Anne Lærke Lorentzen,