Webinar
A step towards national standardization of ctDNA detection
National Infrastructure 2 (NI2) has conducted the second round of a quality assessment trial (EQA) of ctDNA detection with the center's own reference material. Hear about the results from the EQA of ctDNA detection, and take part in the discussion of the next steps to increase the quality and standardisation of ctDNA detection in Denmark.
When?
19 September 2024
15:30 - 16:30
Where?
Online
Link will be provided by mail
Registration
Who?
Anyone interested from the DCCC ctDNA Research Center (this webinar is not for our international partners or the industry).
Seminar leader?
Maria Hønholt Jørgensen
Mads Heilskov Rasmussen
Department of Molecular Medicine
Aarhus Univeristy Hospital
Background
National Infrastructure 2 (NI2) has now completed the second round of a quality assessment trial (EQA) for ctDNA analyses with participating laboratories both nationally and internationally, which routinely analyze low-frequency DNA measurements in 'liquid biopsies' using ddPCR or NGS-based methods.
The goal of the center's ctDNA detection EQA is to create a shared reference framework for assessing the quality of ctDNA detection, including analytical sensitivity, specificity, linearity, and reproducibility, in order to improve and standardize the quality of ctDNA analyses performed in Denmark.
Purpose of the EQA:
1. To bring ctDNA analysis closer to clinical implementation
- Increase harmonization and quality
- Generate knowledge towards establishing a certified standard for ctDNA detection quality in Denmark
2. To develop guidelines for best practices in ctDNA analyses
- Knowledge sharing
- Report results in a scientific article
The center's own reference material has been used in the EQA. The reference material includes both mutations that are central to the development of cancer and mutations that are commonly used drug targets. Additionally, the material spans a broad noise profile and various mutation types.
The reference material is designed to include variants that are frequently analyzed by various ctDNA laboratories and can be analyzed using the most common laboratory methods, including ddPCR and NGS.
In the first EQA round, 7 different national laboratories participated, and more than 60 of the center's reference samples were analyzed using various methods, spanning 6 different ddPCR targets and 4 different NGS assays. The results of the second round are now ready. In the second round, 8 national laboratories and 13 international laboratories participated. A total of 248 reference samples were sent out for analysis using more than 10 different NGS-based methods and 15 different ddPCR targets.
The Webinar
At the webinar, NI2 will talk about the reference material, the results from the two EQA rounds, and initiate a discussion on the structure of future certification, as well as the next steps to improve the quality and standardization of ctDNA detection in Denmark.
We look forward to welcoming you to the webinar!
ADDRESS FOR THE SECRETARIAT
Science Center Skejby, MOMA
Brendstrupgårdsvej 21, build. A
8200 Aarhus N
CONTACT
ctDNA@clin.au.dk
+45 78 45 53 39